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KMID : 0613820050150050819
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Journal of Life Science
2005 Volume.15 No. 5 p.819 ~ p.825
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The Antioxidative Activity of Glutathione-Enriched Extract from Saccharomyces cerevisiae FF-8 in In Vitro Model System
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Lee Chi-Hyeoung
Cha Jae-Young
Jun Bang-Sil
Lee Ho-Joon
Lee Young-Chun
Choi Yong-Lark
Cho Young-Su
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Abstract
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The Antioxidative activities of the cell free extracts containing high glutathione by Saccharomyces cerevisiae FF-8 were tested in vitro experimental models : DPPH method for radical scavenging activity, ferric TBA method and ferric thiocyanate method using linoleic acid and tissue microsome for lipid peroxidation inhibitions. The concentration of intercellular glutathione by cultivating S. cerevisiae FF-8 in the YM optimal medium obtained 204 ㎍/mL, which was increased by 2.76-fold from 74 ㎍/mL in the YM basal medium. A comparition between the YM basal medium and the YM optimal medium on antioxidative substance produced by S. cerevisiae FF-8 was investigated. In DPPH (α,α’-diphenyl-β-picrylhydrazyl) method, the electron donating activity of the glutathione produced by S. cerevisiae FF-8 cultured in the YM optimal medium was as high as that of BHT (0.05%, w/v). The antioxidative activity was measured by inhibition against lipid peroxidation of rat tissues’ microsomes. The results of antioxidant activity of the cell free extracts by S. cerevisiae FF-8 cultured in the YM optimal medium was shown in the following order : liver 60.98%＞kidney 56.43%＞heart 52.91%＞brain 52.13%＞testis 45.57%＞spleen 42.95%. In antioxidative activities determined by ferric thiocyanate method and TBA methods against lipid peroxidation, the lipid peroxidation in the control mixture increased more rapidly than the typical peroxidation curve of linoleic acid from one day. The antioxidative activity of the cell free extracts by cultivating S. cerevisiae FF-8 in the YM optimal medium were higher than that of the YM basal medium. These data indicate that the cell free extracts containing a high intercellular glutathione of S. cerevisiae FF-8 cultured in YM optimal medium showed strong antioxidative capacities by DPPH radical scavenging activity and ferric thiocyanate and TBARS measurements.
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